Part:BBa_K4806210:Design

POR gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1353
  Illegal PstI site found at 2613
  Illegal PstI site found at 2673
  Illegal PstI site found at 3332
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 249
  Illegal NheI site found at 2538
  Illegal PstI site found at 1353
  Illegal PstI site found at 2613
  Illegal PstI site found at 2673
  Illegal PstI site found at 3332
  Illegal NotI site found at 2978
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 530
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1353
  Illegal PstI site found at 2613
  Illegal PstI site found at 2673
  Illegal PstI site found at 3332
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1353
  Illegal PstI site found at 2613
  Illegal PstI site found at 2673
  Illegal PstI site found at 3332
  Illegal NgoMIV site found at 3834
  Illegal NgoMIV site found at 3840
  Illegal NgoMIV site found at 4252
  Illegal AgeI site found at 268
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. AβSAP(i)-promotor (A1-B2) (BBa_K4806013)
• 2. POR gene (B3-B4) (BBa_K4806003)
• 3. FLAG-tag (B5) (BBa_K4806012)
• 4. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B2-B3 (BBa_K3002303)^*
• 2. Connector B4-B5 (BBa_K3002304)^*
• 3. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.